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Journal of Electron Microscopy 24(3): 155-165 (1975)
© 1975 Oxford University Press

Freeze-Fracture Studies of Various Membrane Components Isolated from Tetrahymena pyriformis Cells

Takashi SEKIYA, Yasuo KITAJIMA and Yoshinori NOZAWA

Department of Biochemistry, Gifu University School of Medicine Tsukasamachi-40, Gifu, Japan

Various subcellular membrane fractions are isolated from a ciliated protozoan Tetrahymena pyriformis WH-14 and examined by freeze-frac-ture electron microscopy. The size and distribution density of membrane-intercalated partides are different between the types of the membrane. The particles of the alveolar membranes (AM) are larger (115±25 Å) than those of the plasma membrane (70±20 Å). The A faces of the distal portion of the ciliary membrane reveal fewer particles as compared to those of the proximal portion.

Received     17 April 1975,
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