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Journal of Electron Microscopy 42(2): 121-123 (1993)
© 1993 Oxford University Press

Imaging of the Surface Structures of Epon Thin Sections Created with a Glass Knife and a Diamond Knife by the Atomic Force Microscope

Kazunobu Amako, Akemi Takade, Akiko Umeda and Masaru Yoshida*

*Hitachi Instruments Service Co.Ltd. Tokyo, 151 Japan
Department of Bacteriology, Faculty of Medicine, Kyushu University Fukuoka, 812 Japan

The surface of Epon thin sections of bacterial cells were imaged with an atomic force microscope. The sectioned surface created by a diamond knife was found to be smoother than that made by a glass knife. The bacterial shape embedded in epoxy resin can be clearly seen on a diamond knife section but not on a glass knife section. The contour of the embedded bacterial cell was imaged as an engraved shape in the Epon matrix. The depth of the engraving was about 6 nm on average measured at the cell wall ridge in the diamond knife section and 30nm in the glass knife section.

Keywords     AFM, thin section, diamond knife, glass knife

Received      9 November 1992, accepted 28 December 1992


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