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Journal of Electron Microscopy 43(6): 367-372 (1994)
© 1994 Oxford University Press

Experimental in vivo and in vitro Formation of Type VI Collagen Periodic Fibrils in Chorionic Villi of Human Placenta

Ken-ichi Iida1,3, Miya Kobayashi2, Kunihiko Kobayashi4, Shinsuke Saga1, Takeshi Hoshino2 and Mutsushi Matsuyama1

1Department of Pathology, Nagoya University School of Medicine Tsurumai-cho 65, Showa-ku, Nagoya, 466 Japan
2Department of Anatomy, Nagoya University School of Medicine Tsurumai-cho 65, Showa-ku, Nagoya, 466 Japan
3Department of Clinical Research Chubu National Hospital
4Nagoya University College of Medical Technology

The distribution of type VI collagen in the chorionic villi of normal human term placenta was studied. Since 100 nm periodic fibrils (type VI collagen periodic fibrils) are known to form in the tendon, cornea and cultured fibroblasts by Incubation with acidic ATP solution, we incubated the placental tissue with 20 mM ATP, fixed it with Karnovsky's fixative and examined under an electron microscope. Type VI collagen periodic fibrils widely occurred in the interstitial connective tissue, especially close to the basal lamina. By cryo-immunoelectron microscopy, labeling for collagen type VI was found in the area between the trophoblastic basal lamina and the endothelial basal lamina. Furthermore, we extracted type VI collagen from the human placenta and brought both extract and residues into ATP treatment. Periodic structure formation was rare in extracted preparation compared with residues. The results served to clarify the mechanism of fibril formation in the tissue.

Keywords     type VI collagen, placenta, ATP, basal lamina, connective tissue

Received      5 July 1994, accepted 7 November 1994


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