Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Sato, S.
Right arrow Articles by Yano, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sato, S.
Right arrow Articles by Yano, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Electron Microscopy 43(6): 373-377 (1994)
© 1994 Oxford University Press

Ultrastructural Localization of Intranucleolar DNA in Vicia faba Root-Tip Cells by Immunocytochemistry Using an Anti-BUdR Antibody

Seiichi Sato and Hiroyuki Yano

Department of Biology, Faculty of Science, Ehime University Bunkyo-cho 2-5, Matsuyama, Ehime, 790 Japan

Ultrastructural localization of intranucleolar DNA in Vicia faba root-tip cells was investigated by an immunocytochemical method consisting of in vitro bromodeoxyuridine (BUdR)-labelling with the aid of terminal deoxynucleotidyltransferase and successive immunocytochemistry using an anti-BUdR antibody. The nucleolus usually had many low electron-opaque patches (LEPs). The fibrillar centers (FCs) occupied their peripheral region leaving a central electron-transparent space (central space). The immunoelectron microscopic technique showed that in the nucleolus the label was mostly confined to the FCs whilst the central space was completely free from the label. In other nucleolar regions the label appeared to gradually increase towards the FCs. In addition to nucleoli, this method also localized a small amount of DNA in mitochondria.

Keywords     BUdR, immunocytochemistry, intranucleolar DNA, terminal deoxynucleotidyltransferase

Received     18 July 1994, accepted 26 October 1994


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.