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Journal of Electron Microscopy 43(6): 394-397 (1994)
© 1994 Oxford University Press

Ultrastructural and Immunocytochemical Study of the Subepithelial Fiber Component of the Guinea Pig Inner Ear

Hidemi Kaname, Toshio Yoshihara, Tetsuo Ishii, Hozumi Tatsuoka* and Tanemichi Chiba*

*Department of Anatomy, Chiba University School of Medicine 1-8-1 lnohana, Chuo-ku, Chiba. 260 Japan
Department of Otolaryngology, Tokyo Women's Medical CoUege 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162 Japan

The connective tissues of the lateral cochlear wall and semicircular canal of the guinea pig were investigated by transmission electron microscopy, intermediate voltage electron microscopy and immunocytochemistry. In the spiral ligament, numerous fibroblasts and fiber bundles were intermingled with each other. Fibroblasts and fibers were also observed in the semicircular canal. Ultrastructurally, immuno-gold particles, indicating the presence of type II collagen, were found on the fibers in the spiral ligament and the semicircular canals. The present results revealed that both stria vascularis and the membranous semicircular canal were supported by a network of fibroblasts and type n collagen fibers.

Keywords     type II collagen, immunocytochemistry, fibroblast, inner ear

Received      6 June 1994, accepted 20 October 1994


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