Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Bhakat, P.
Right arrow Articles by Bhattacharya, D.K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bhakat, P.
Right arrow Articles by Bhattacharya, D.K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Electron Microscopy 46(1): 59-66 (1997)
© 1997 Oxford University Press

Image contrast of biological materials in high-voltage electron microscopy

P. Bhakat and D.K. Bhattacharya*

Department of Biochemistry and Biophysics, University of Kalyani Kalyani-741235, India

*To whom correspondence should be addressed

A new and simple expression has been formulated for the estimation of contrast of amorphous specimens in the tilted-beam mode of dark-field (DF) imaging in a high-voltage electron microscope. DF contrasts have been calculated for AIDS1.DNA, Salmonella typhimurium DNA,human haemoglobin and myoglobin of sperm whale in the range 0.1–3.0 MV and compared with the corresponding bright-field (BF) contrasts. DF contrast is seen to be significantly greaterthan BF contrast for a wide range of microscope apertures ({alpha}) and voltages (ø). The ratio of DF to BF contrast was observed to increase with increasing ø and {alpha} for all the aforesaid specimens.

Keywords     high-voltage electron microscopy,, image contrast,, dark-field,, tilted-beam imaging,, biological specimens

Received      5 February 1996, accepted 1 September 1996


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.