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Journal of Electron Microscopy 47(4): 351-354 (1998)
© 1998 Oxford University Press

Low-voltage backscattered electron imaging of non-coated biological samples in a low-vacuum environment using a variable-pressure scanning electron microscope with a YAG-detector

Tatsuo Ushiki*,3, Hiroya Hashizume3, Sukehiro Itoh1, Kenzo Kuboki1, Syobu Saito1 and Keiichi Tanaka2

1Hitachi Science Systems, Ltd Hitachinaka 312
2Seirei Christopher College of Nursing Hamamatsu 433, Japan
3Department of Anatomy, Niigata University School of Medicine Niigata 951

*To whom correspondence should be addressed. E-mail: t-ushiki{at}med.niigata-u.ac.jp

A variable-pressure scanning electron microscope (VP-SEM) equipped with a high-sensitive backscattered electron (BSE) detector of the YAG type was applied to studies of biological tissue samples. The rat kidney and trachea were fixed, dehydrated in ethanol, critical point-dried and examined in the vp-sem under a specimen chamber pressure of 1 to 150 Pa. The high-resolution surface images of the non-coated specimens were obtained in a low-vacuum (1–20 Pa) environment at an accelerating voltage of 5 kV, while the images at 10–20 kV contained information beneath the surface of the specimens. The observation in the VP-SEM equipped with YAG detector in a low-vacuum (1–20 Pa) environment at low (3–5 kV) accelerating voltages is useful for the three-dimensional analysis of the surface morphology of biological non-coated samples.

Keywords     backscattered electron (BSE) imaging, variable pressure scanning electron microscope, low-vacuum, low voltage, Yttrium Aluminum Garnet (YAG) detector, biology

Received      5 March 1998, accepted 19 May 1998


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