Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Terada, N.
Right arrow Articles by Ohno, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Terada, N.
Right arrow Articles by Ohno, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Electron Microscopy 47(5): 489-493 (1998)
© 1998 Oxford University Press

Scanning electron microscopic study of erythrocyte shapes artificially jetted through tubes at different pressures by ‘in vitro cryotechnique for erythrocytes’

Nobuo Terada*, Yasuhisa Fujii, Yasuko Kato, Hideho Ueda, Takeshi Baba and Shinichi Ohno

Department of Anatomy, Yamanashi Medical University 1110 Shimokato, Tamaho, Yamanashi 409-3898, Japan

*To whom correspondence should be sent

A new cryotechnique for examining morphological changes of human erythrocytes at different jet pressures was developed in the present study. Human fresh or stored erythrocytes passing through a tube were jetted into precooled isopentane-propane mixture (–193°C), which was named as ‘in vitro cryotechnique for erythrocytes’. After the cryotechnique procedure, the routine freeze-substitution method and subsequent t-butyl alcohol freeze-drying method were used for preparing the scanning electron microscopic specimens. At 100 mmHg or higher pressures of jetting, lamellar-arranged erythrocytes were observed to have elongated shapes. The more the jetting pressure increased, the fewer discoid shapes of erythrocytes were observed. This cryofixation technique could preserve the morphology of erythrocytes jetting from tubes, and provide the three-dimensional image of erythrocyte surfaces, as followed by scanning electron microscopy.

Keywords     in vitro cryotechnique, erythrocyte shape, freeze-substitution, jet-freezing, jetting pressure

Received      6 August 1997, accepted 26 May 1998


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.