Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Sawada, H.
Right arrow Articles by Esaki, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Sawada, H.
Right arrow Articles by Esaki, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Electron Microscopy 47(5): 535-537 (1998)
© 1998 Oxford University Press

A simple flat embedding method for the correlative light and electron microscopic immunocytochemistry

Hajime Sawada* and Michiyo Esaki

Department of Anatomy, Yokohama City University, School of Medicine Fukuura 3-9, Kanazawa-ku, Yokohama 236-0004, Japan

*To whom correspondence should be addressed. E-mail: hsawada{at}yellow.med.yokohama-cu.ac.jp

For correlative light and electron microscopic immunocytochemistry, a simple method for flat embedding of cryostat sections in Epon was devised. In the present technique tissue sections were attached to widely used silane-coated slides, processed, and embedded in Epon beneath non-adhesive transparent plastic films. The Epon block can be easily removed from slides by heating. This method simultaneously offers transparency and thinness of the embedded blocks, absolute flatness of the sections, and easy removal of Epon from embedding molds.

Keywords     embedding, Aclar film, OHP film, Epon, immunocytochemistry, immunoelectron microscopy

Received     24 February 1998, accepted 30 June 1998


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
H. Sawada and M. Esaki
A Practical Technique to Postfix Nanogold-immunolabeled Specimens with Osmium and to Embed Them in Epon for Electron Microscopy
J. Histochem. Cytochem., April 1, 2000; 48(4): 493 - 498.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.