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Journal of Electron Microscopy 47(5): 539-542 (1998)
© 1998 Oxford University Press

Colour scanning electron microscopy of peltate glandular trichomes of fresh developing hops (Humulus lupulus L.)

Mitsuhiko Yamada1,*, Takeo Suzuki1, Eisaku Oho2 and Hisashi Matsushima3

1Hitachi Instruments Engineering Co. Ltd 882 Ichige Hitachinaka, Ibaraki 312, Japan
2Department of Electrical Engineering, Kogalcuin University 1-24-2 Nishishinjuku, Shiinjuku-ku, Tokyo 163-91, Japan
3Department of Regulation-Biology, Faculty of Science, Saitama University 255 Shimookubo, Urawa, Saitama 338, Japan

*To whom correspondence should be addressed. E-mail: yamada_hk{at}cm.naka.hitachi.co.jp

Morphological and colour changes of fresh developing hop female cones were examined using a colour scanning electron microscopy technique. A highlight component of a video microscope image was replaced with the counterpart of an SEM image by digital image processing for better resolution. In order to obtain colour information specific to specimen surfaces, the SEM observation was carried out under low vacuum ({small tilde}50 Pa) and at a low temperature ({small tilde}20°C) without conventional specimen preparations. It was found that the bottom cuticular surface of the cup-shaped trichomes gradually upheaved and their colour turned yellow, as the cones grew.

Keywords     colour scanning electron microscopy, trichome of hop, low-vacuum SEM, highlight component, video microscope, digital image processing

Received     24 February 1998, accepted 7 July 1998


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