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Journal of Electron Microscopy 48(1): 85-87 (1999)
© 1999 Oxford University Press

Detection of glycoconjugates by lectin gold labelling, silver enhancement and scanning electron microscopy

Andreas Gebert*,, Susanne Faßbender, Annikka Weißerdt and Gerd Preiss1

Department of Functional and Applied Anatomy, Medical School of Hannover D-30623 Hannover, Germany
1Department of Cell Biology in the Centre of Anatomy, Medical School of Hannover D-30623 Hannover, Germany

*To whom correspondence should be addressed

A method for detecting glycoconjugates on cell surfaces in scanning electron microscopy is described. Terminal saccharides were specifically recognized by a lectin conjugated to biotin, and, after incubation with an anti-biotin antibody conjugated to colloidal gold, silver enhancement was used to produce deposits large enough to be detected in standard scanning electron microscopes. Secondary electron images revealed the ultrastructure of the tissue investigated, while backscattered electron images showed the distribution of lectin binding sites. Using digital recording and processing, the two channels were combined in colour-encoded images. The new method brings together lectin histochemistry and scanning electron microscopy and thus allows the three-dimensional distribution of glycoconjugates to be analysed at an ultrastructural level.

Keywords     lectin, colloidal gold, scanning electron microscopy, silver enhancement, backscattered electrons, digital image processing

Received     30 July 1998, accepted 13 October 1998


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