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Journal of Electron Microscopy 48(4): 449-454 (1999)
© 1999 Oxford University Press
Structural injury of osteosarcoma mitochondria by a novel antitumour agent, 2-methylfuranonaphthoquinone
1Department of Anatomy, Kanazawa Medical University Uchinada, Ishikawa 920-0293, Japan
2Kobe Pharmaceutical University Higashinada, Kobe 658-8558, Japan
*To whom correspondence should be addressed. E-mail: keih{at}kanazawa-med.ac.jp
The effect of the novel anticancer 2-methylnaphtho[2,3-b]furan-4,9-dione (FNQ3) on human osteosarcoma cell lines (HuO9 and HuO9N2) was investigated. The IC50 values of FNQ3 were 5.95 µM for HuO9 and 3.86 µM for HuO9N2, while that for normal fibroblasts (WI-38 cell line) was 35.8 µM. The selectivity in antitumour activity which was estimated from the IC50 ratio of normal fibroblasts to tumour cells was 6.0 and 9.3 fold for HuO9 and HuO9N2, respectively. FNQ3 at 23.6 µM selectively injured mitochondria of HuO9 cells starting at 36 h and HuO9N2 cells at 24 h, whereas WI-38 cells were unaffected even after 72 h. These results demonstrated that FNQ3 was selectively toxic to the mitochondria of osteosarcoma cells similar to carcinoma cells (Pan et al. (1997) J. Electron Microsc. 46: 181), in comparison to normal cells.
Keywords furanonaphthoquinone, human osteosarcoma, HuO9 cell line, HuO9N2 cell line, growth-inhibition toxicity, anticancer drug
Received 29 September 1998, accepted 28 April 1999