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Journal of Electron Microscopy 48(5): 665-669 (1999)
© 1999 Oxford University Press

The advantage of the osmium conductive metal coating for the detection of the colloidal gold-conjugated antibody by SEM

Tokuji Osawa1,*, Yasuo Yoshida2, Fumio Tsuzuku1, Momoki Nozaka1, Minoru Takashio2 and Yohichiro Nozaka1

1Oral Anatomy I, Iwate Medical University School of Dentistry
2Laboratory for Electron Microscopy, Iwate Medical University Morioka, Iwate 020–8505, Japan

*To whom correspondence should be addressed. E-mail: tosawa{at}iwate-med.ac.jp

For the detection of the colloidal gold-conjugated antibody by scanning electron microscopy, the comparison between the secondary and the back-scattered electron images is necessary. After the various durations of osmium conductive metal coatings, enlargements of the diameters of the antibodies were observed. The thick coating reduced the contrast of the backscattered electron images. By the optimal thickness of the coating, the secondary electron image showed a minimum reduction in the resolution, and the backscattered electron image showed not only the gold particles with high contrast, but also the outline of the basic morphology.

Keywords     osmium conductive metal coating, colloidal gold-conjugated antibody, secondary electron image, backscattered electron image

Received     18 March 1999, accepted 24 June 1999


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