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Journal of Electron Microscopy 49(1): 195-202 (2000)
© 2000 Oxford University Press

Scanning electron microscopy subsequent to a combined treatment of NaOCl and EDTA in some non-collagenous calcified matrixes

Tetsuo Kodaka1,*, Tsuneyoshi Sano2 and Ryoichi Mori2

1Second Departments of Oral Anatomy, Showa University School of Dentistry 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
2First Departments of Oral Anatomy, Showa University School of Dentistry 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan

*To whom correspondence should be addressed

Using backscattered electron (BSE) imaging and scanning electron microscopy, subsequent to a combined treatment of sodium hypochlorite (NaOCl) and ethylenediamine tetra-acetic acid (EDTA) or only with EDTA etching, we observed some structures of non-collagenous calcified matrixes with the aim of revealing the correlation of deposition between calcification degree and organic amount. In human tooth enamel, the NaOCl-EDTA method eroded more intensively the hypocalcified prisms of enamel tufts containing a relatively large amount of EDTA-insoluble organic matter than the hyper-calcified normal prismatic enamel containing a small amount of the organic matter. Afibrillar cementum, one of the non-collagenous calcined tissues similar to the enamel, has been reported to consist of organic-rich and poor incremental lamellae. The BSE imaging showed an alternation pattern of hypocalcification and hypercalcification. The hypocalcified lamellae were retained by EDTA etching, while the hypercaldfied lamellae showed a resistance against the NaOCl-EDTA method. In the non-collagenous calcareous concretions of human pineal body, organic-rich and poor, and hyper- and hypocalcified incremental lamellae have been reported. The deposition pattern of calcification degree and organic amount was similar to that in afibrillar cementum, and the hypercaldfied lamellae showed a resistance against the NaOCl-EDTA method. In conclusion, the nigh and the lower caldfied regions of non-collagenous calcified matrixes contained smaller and larger amounts of EDTA-insoluble organic matter respectively. Moreover, scanning electron microscopy subsequent to the NaOCl-EDTA method corresponding to the BSE imaging clearly showed fine caldfied structures compared with the BSE imaging.

Keywords     non-collagenous calcified matrix, backscattered electron imaging, scanning electron microscopy, calcification degree, organic amount

Received     22 July 1999, accepted 8 November 1999


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