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Journal of Electron Microscopy 52(6): 581-591 (2003)
© Japanese Society of Microscopy

Full-length paper

Possible role of immunocompetent cells and the expression of heat shock protein-25 in the process of pulpal regeneration after tooth injury in rat molars

Kuniko Nakakura-Ohshima1, Jun-ichi Watanabe2, Shin-ichi Kenmotsu3 and Hayato Ohshima3,*

1 Polyclinic Intensive Oral Care Unit, Niigata University Medical and Dental Hospital, 2 Division of Pediatric Dentistry, Department of Oral Health Science and 3 Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Niigata 951-8514, Japan

* To whom correspondence should be addressed. E-mail: histoman{at}dent.niigata-u.ac.jp

Abstract

Recent studies have established that heat shock proteins (HSPs) potentially play a role in immunosurveillance. The purpose of the present study was to clarify the relationship between the chronological changes of immunocompetent cells and the expression of HSP-25 in the process of pulpal regeneration after tooth injury in rat molars by immunocytochemistry for HSP-25 and class II major histocompatibility complex (MHC) antigen. In untreated control teeth, intense HSP-25 immunoreactivity was found in the cell bodies of odontoblasts. Both cavity preparation and tooth replantation caused the degeneration of the odontoblast layer to result in the loss of HSP-25 immunoreactions in the suffered dental pulp at the early stages after tooth injury. Numerous class II MHC-positive cells appeared along the pulp–dentin border and extended their cell processes into the dentinal tubules at 12–24 h after cavity preparation and 3 days after tooth replantation. Newly differentiated odontoblast-like cells with HSP-25 immunoreactivity were arranged at the pulp–dentin border and the class II MHC-positive cells retreated towards the subodontoblastic layer by post-operative days 3–5 after tooth injury. Thus, the common cellular events occur during pulpal regeneration following two different experimental injuries. These findings indicate that the time course of changes in the expression of HSP-25 immunoreactivity reflects the degeneration/regeneration process of odontoblasts and that the temporal appearance of the class II MHC-positive cells at the pulp–dentin border suggests their participation in odontoblast differentiation as well as in initial defence reactions during the pulpal regeneration process.

Keywords     class II MHC-positive cells, heat shock protein-25, odontoblasts, pulpal regeneration, cavity preparation, tooth replantation

Received      4 August 2003, accepted 17 September 2003


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Y. Takamori, H. Suzuki, K. Nakakura-Ohshima, J. Cai, S.-W. Cho, H.-S. Jung, and H. Ohshima
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J. Histochem. Cytochem., December 1, 2008; 56(12): 1075 - 1086.
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