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Journal of Electron Microscopy Advance Access originally published online on August 25, 2005
Journal of Electron Microscopy 2005 54(4):317-324; doi:10.1093/jmicro/dfi049
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© The Author 2005. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Transfer doublet and an elaborated phase plate holder for 120 kV electron-phase microscope

Fumio Hosokawa1, Radostin Danev2,*, Yoshihiro Arai1 and Kuniaki Nagayama2

1 JEOL Ltd, 3-1-2 Musashino, Akishima, Tokyo 196-8558, Japan and 2 Center for Integrative Bioscience, Okazaki National Research Institutes, Myodaiji-cho, Okazaki 444-8585, Japan

* To whom correspondence should be addressed. E-mail: rado{at}nips.ac.jp

A Zernike-type phase plate, which consisted of an amorphous carbon film, was developed for a 120 kV transmission electron microscope (TEM). The thickness of the film causes the scattered electrons to experience a {pi}/2 phase shift. The axial electrons pass through the central hole of the phase plate. The TEM was modified with an additional lens doublet, which allowed more flexibility in applying the phase plates. The doublet transfers the image from the back focal plane to a plane below the objective lens where a special phase plate holder, anticontaminator, and other necessary devices could be employed. The diameter of the central hole in the phase plate was 1 µm. The same mechanical system as in the specimen stage is used to align the axial beam into the hole. The use of a heating holder to protect the phase plate from contamination and consequential charging was investigated. Comparative diffractograms of amorphous carbon are shown with and without using a phase plate, demonstrating the change in phase contrast modulation from a sine to a cosine function when a phase plate is used. Images of biological specimens acquired using a phase plate are presented and the contrast enhancement due to the cosine transfer function is clearly demonstrated.

Keywords     transfer lens, phase plate, CTF, phase contrast

Received      1 March 2005, accepted 3 June 2005


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K. Nagayama and R. Danev
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[Abstract] [Full Text] [PDF]


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