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Journal of Electron Microscopy Advance Access originally published online on August 2, 2005
Journal of Electron Microscopy 2005 54(4):379-383; doi:10.1093/jmicro/dfi047
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© The Author 2005. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Micro-CT of Pseudocneorhinus bifasciatus by projection X-ray microscopy

Akira Tanisako1, Ayumi Hori1, Aya Okumura1, Chikara Miyata1, Chiaki Kuzuryu1, Takashi Obi2 and Hideyuki Yoshimura1,*

1 Meiji University, Department of Physics, 1-1-1 Higashimita, Tama-ku, Kawasaki 214-8571, Japan and 2 Tokyo Institute of Technology, Inter Disciplinary Graduate School of Science and Engineering, Department of Information Processing, 4259 Nagatsuda, Midori-ku, Yokohama 226-8503, Japan

* To whom correspondence should be addressed. E-mail: hyoshi{at}isc.meiji.ac.jp

The projection X-ray microscope utilises a very small X-ray source emitted from a thin (0.1–3 µm) target metal film excited by the focused electron beam of a scanning electron microscope. When an object is placed just below the target metal film, the diverging X-rays enlarge the shadow of the object. Because no X-ray optics such as a zone-plate is used, the focal depth is, in principle, infinitely large. We exploited this to apply projection X-ray microscopy to three-dimensional (3-D) structure analysis by means of cone-beam computed tomography. The projection images of a small arthropod (Pseudocneorhinus bifasciatus, 5 mm in length), was recorded at 3° increments over the whole range (360°) of a stepping-motor-controlled sample rotator. A 3-D image was reconstructed from corn-beam projections using a filtered back-projection algorithm. The reconstructed 3-D image showed in detail the internal structure of an opaque object.

Keywords     X-ray microscopy, computer tomography, CT, projection X-ray microscope, arthropod, internal structure

Received     14 April 2005, accepted 13 May 2005


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