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Journal of Electron Microscopy Advance Access originally published online on January 12, 2007
Journal of Electron Microscopy 2007 56(1):1-5; doi:10.1093/jmicro/dfl039
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Electron holography on dynamic motion of secondary electrons around sciatic nerve tissues

Daisuke Shindo1,*, Joong Jung Kim1, Weixing Xia1, Ki Hyun Kim1, Nobuhiko Ohno2, Yasuhisa Fujii2, Nobuo Terada2 and Shinichi Ohno2

1 Institute of Multidisciplinary Research for Advanced Materials, Tohoku University Sendai 980-8577
2 Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi Chuo-shi 409-3898, Japan

*To whom correspondence should be addressed. E-mail: shindo{at}tagen.tohoku.ac.jp

By means of electron holographic visualization of detailed electric potential distribution around sciatic nerve tissues coated with C and OsO4, we show that the steady state of these specimens subjected to intense charging with electron irradiation is accompanied with a dynamic motion of collective secondary electrons; the secondary electrons emitted from the coated specimens revolve around the positively charged specimens forming stationary orbits. Further, this study clarified the possibility of the direct visualization of a part of the orbits of the collective secondary electrons without disturbing their motions.

Keywords     electron holography, secondary electron emission, charging effect, electric potential distribution, sciatic nerve tissue, microfibril

Received      8 November 2006, accepted 12 December 2006


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Comment on 'Electron holography on dynamic motion of secondary electrons around sciatic nerve tissues'
J. Electron Microsc. (Tokyo), October 1, 2008; 57(5): 165 - 167.
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