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Journal of Electron Microscopy Advance Access originally published online on April 22, 2009
Journal of Electron Microscopy 2009 58(5):305-313; doi:10.1093/jmicro/dfp019
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Three-dimensional structure of the cytoskeleton in Trichomonas vaginalis revealed new features

Kyung Eun Lee1, Ji Hui Kim1, Min Kyo Jung1, Tatsuo Arii2, Jae-Sook Ryu3 and Sung Sik Han1,*

1 School of Life Sciences and Biotechnology, Korea University, 1, 5 Anamdong, Seongbukgu, Seoul, Korea
2 National Institute for Physiological Sciences, 38 Nishigonaka Myodaiji, Okazaki, Aichi, 444-8585, Japan
3 Department of Parasitology, College of Medicine, Hanyang University, 17, Haengdangdong, Seongdonggu, Seoul, Korea

* To whom correspondence should be addressed. E-mail: sshan{at}korea.ac.kr

The flagellated protozoan Trichomonas vaginalis has been widely studied owing to its medical significance and unique structure. The complicated three-dimensional (3D) structure of the cellular components of T. vaginalis was reconstructed from serial sections to enable observation of the spatial features of the whole cell. Electron tomography was used to examine the detailed structure of the cellular organelles. Tomographic reconstruction showed the mastigont system and the parabasal filament of T. vaginalis in detail. The last thin filament (Pf3) was located close to the adjacent filament, and the two filaments appeared to be vertically parallel in the cross-sectional view. It is likely that Pf3 cannot be distinguished from the adjacent filament in 2D images obtained from transmission electron microscopy. Our 3D reconstruction of T. vaginalis revealed the presence of an additional striated fiber, and 3D reconstruction by electron tomography showed twisting of the split parabasal filament.

Keywords     T. vaginalis, 3D structure, electron tomography, flagellar beating, additional parabasal filament, twisting structure

Received     30 January 2009, accepted 23 March 2009


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