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Journal of Electron Microscopy Advance Access originally published online on June 12, 2009
Journal of Electron Microscopy 2009 58(6):363-373; doi:10.1093/jmicro/dfp029
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy

Ryuta Kaneko1,2, Hirotoshi Akita2, Hidetoshi Shimauchi1 and Yasuyuki Sasano2,*

1 Division of Periodontology and Endodontology
2 Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan

* To whom correspondence should be addressed. E-mail: sasano{at}anat.dent.tohoku.ac.jp

STRO-1 is a cell-surface antigen. A cell population that is positive for the anti-STRO-1 antibody has been shown to contain mesenchymal stem cells. STRO-1-positive cells have been reported to reside in dental pulp and periodontal ligaments as well as in bone marrow. However, the tissue localization of STRO-1 in developing teeth is not clear. The present study was designed to investigate the spatiotemporal localization of STRO-1 in developing rat teeth by immunohistochemistry using light microscopy and electron microscopy. Wistar rats at ages 2, 3, 6 and 12 weeks postnatum and embryos at 18 days postcoitum were fixed with 4% paraformaldehyde. Mandibles and maxillae were resected and decalcified in 10% EDTA. The specimens were embedded in paraffin, and sections were cut and processed for immunohistochemistry using the anti-STRO-1 antibody. Selected specimens were frozen, sectioned and processed for immunoelectron microscopy. Immunoreactions for STRO-1 were identified in some bone marrow cells. Some odontoblasts and dental pulp cells showed positive immunoreactivity in developing rat tooth crowns and roots. Alveolar osteoblasts, cementoblasts and periodontal ligament cells were also immunoreactive. Electron microscopy localized the antigen in plasma membrane and some vesicles in dental pulp cells and odontoblasts. The study suggests that the STRO-1 antigen is involved in the differentiation of mesenchymal cell lineages and formation of the matrix in dental tissues.

Keywords     STRO-1, tooth, development, immunohistochemistry, immunoelectron microscopy, rats

Received     17 March 2009, accepted 7 May 2009


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