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Journal of Electron Microscopy Advance Access published online on August 1, 2009
Journal of Electron Microscopy, doi:10.1093/jmicro/dfp039
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Old age is associated with ultrastructural changes in isolated rat liver sinusoidal endothelial cells

Jennifer N. O’Reilly, Victoria C. Cogger and David G. Le Couteur*

Centre for Education and Research on Ageing and ANZAC Research Institute, University of Sydney and Concord RG Hospital, Sydney 2139, Australia

* To whom correspondence should be addressed. E-mail: dlecouteur{at}med.usyd.edu.au

Old age is associated with ultrastructural changes in the hepatic sinusoid called pseudocapillarization, which include defenestration and thickening of the sinusoidal endothelium. We investigated whether such changes also occur in isolated and cultured liver sinusoidal endothelial cells. Liver sinusoidal endothelial cells were isolated from young (6–10 months, n = 4) and old (24–26 months, n = 4) F344 rats and fenestrations evaluated using scanning electron microscopy. Fenestration diameter was reduced in old age from 194 ± 1 nm to 185 ± 1 nm (P < 0.001) and there was an age-related increase in the number fused fenestrations and large gaps. Age-related changes in the diameter of fenestrations in the liver sinusoidal endothelial cell are maintained following isolation and culture. This suggests that this age-related change may be intrinsic to the liver endothelial cell and/or irreversible.

Keywords     sinusoidal endothelial cell, fenestration, aging

Received     30 April 2009, accepted 7 July 2009


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