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Journal of Electron Microscopy Advance Access published online on August 8, 2009
Journal of Electron Microscopy, doi:10.1093/jmicro/dfp040
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Cyanoacrylate glue as an alternative mounting medium for resin-embedded semithin sections

Pei-Yun Liu1,*, Gael E. Phillips2, Margit Kempf1, Leila Cuttle1, Roy M. Kimble1 and James R. McMillan1

1 Burns and Trauma Research Group, Queensland Children Medical Research Institute, Department of Paediatrics and Child Health, The University of Queensland
2 Department of Anatomical Pathology, Pathology Queensland, Royal Brisbane and Women's Hospital, Brisbane, Australia

* To whom correspondence should be addressed. E-mail: p.liu{at}uq.edu.au

Commercially available generic Superglue (cyanoacrylate glue) can be used as an alternative mounting medium for stained resin-embedded semithin sections. It is colourless and contains a volatile, quick-setting solvent that produces permanent mounts of semithin sections for immediate inspection under the light microscope. Here, we compare the use of cyanoacrylate glue for mounting semithin sections with classical dibutyl phthalate xylene (DPX) in terms of practical usefulness, effectiveness and the quality of the final microscopic image.

Keywords     cyanoacrylate glue, mounting medium, semithin sections, dibutyl phthalate xylene, transmission electron microscopy, microscopic image

Received     26 June 2009, accepted 10 July 2009


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