Journal of Electron Microscopy 32(2): 125-140 (1983)
© 1983 Oxford University Press
Release of Vaccinia Virus from FL Cells Infected with the IHD-W Strain
Department of Virology, Okayama University Medical School Shikatacho, Okayama, 700 Japan
To study the mechanism of release of vaccinia virus from host cells, FL cells infected with the IHD-W strain of vaccinia virus were examined through one growth cycle by scanning and transmission electron microscopy (SEM and TEM). Brick-shaped virions appeared on the surface of infected cells with in 8 hr of infection and increased in number as infection proceeded. The budding of virions from the cellular surface was detected 12 hr after infection, and thereafter until the end of the experiment. This budding process was seen more frequently at later stages of infection (16–48 hr). Besides the budding of virions, another mode of virus release, i.e., reverse phagocytosis of double-membraned virions, was also revealed. Plasma membranes of FL cells at the late stage of infection were examined by SEM as well as by TEM, and it was shown that numerous naked virions were intimately associated with the inner face of the membranes.
Keywords vaccinia virus release, vaccinia budding, virus-plasma membrane association, scanning electron microscopy (SEM), transmission electron microscopy (TEM)
Received 10 January 1983, accepted 6 April 1983
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