Journal of Electron Microscopy 35(3): 304-306 (1986)
© 1986 Oxford University Press
A Stable Lead by Modification of Sato's Method
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1Central Laboratory for Electron Microscopy, Nagoya University School of Medicine Showa-ku, Nagoya, 466 Japan
2Electron Microscopic Laboratory, School of Medicine, Fujita-gakuen Health University Toyoake, Aichi, 470-11 Japan
3Department of Pathology, Nagoya University School of Medicine Showa-ku, Nagoya, 466 Japan
4Katayama Chemical Industry Co. Ltd. Osaka, 541 Japan
We modified Sato's lead stain in order to obtain a more stable staining solution. The staining solution formed no lead carbonate film on the surface for periods up to 3 hr after exposure to the air. No precipitates were formed in the solution kept at room temperature for over 1 year. The best results were obtained when Epon sections were stained with the new stain for 2 min.
Keywords electron stain, lead staining solution, Sato's method, calcined lead citrate, long-term storage lead solution
Received 12 August 1986, accepted 26 November 1986
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