Journal of Electron Microscopy 52:161-174 (2003)
© 2003 Oxford University Press
Ultrastructure and behavior of actin cytoskeleton during cell wall formation in the fission yeast Schizosaccharomyces pombe
1Division of Material and Biological Sciences, Graduate School of Science and 2Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan
*To whom correspondence should be addressed. E-mail: osumi{at}fc.jwu.ac.jp
Fluorescence microscopy has shown that F-actin of the fission yeast Schizosaccharomyces pombe forms patch, cable and ring structures. To study the relationship between cell wall formation and the actin cytoskeleton, the process of cell wall regeneration from the protoplast was investigated by transmission electron microscopy (TEM), immunoelectron microscopy (IEM) and three-dimensional reconstruction analysis. During cell wall regeneration from the protoplast, localization of F-actin patches was similar to that of the newly synthesized cell wall materials, as shown by confocal laser scanning microscopy (CLSM). In serial sectioned TEM images, filasomes were spherical, 100–300 nm in diameter and consisted of a single microvesicle (35–70 nm diameter) surrounded by fine filaments. Filasomes were adjacent to the newly formed glucan fibrils in single, cluster or rosary forms. By IEM analysis, we found that colloidal gold particles indicating actin molecules were present in the filamentous area of filasomes. Three-dimensional reconstruction images of serial sections clarified that the distribution of filasomes corresponded to the distribution of F-actin patches revealed by CLSM. Thus, a filasome is one of the F-actin patch structures appearing in the cytoplasm at the site of the initial formation of the cell wall and it may play an important role in this action.
Keywords actin cytoskeleton, filasome, cell wall formation, three-dimensional reconstruction, Schizosaccharomyces pombe, immunoelectron microscopy
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Yamaguchi, H. Okada, and Y. Namiki Smart specimen preparation for freeze substitution and serial ultrathin sectioning of yeast cells J. Electron Microsc. (Tokyo), August 1, 2009; 58(4): 261 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Iwaki, T. Sano, T. Takagi, M. Osumi, A. Kihara, and Y. Igarashi Intracellular Trafficking Pathway of Yeast Long-chain Base Kinase Lcb4, from Its Synthesis to Its Degradation J. Biol. Chem., September 28, 2007; 282(39): 28485 - 28492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Osumi, M. Konomi, T. Sugawara, T. Takagi, and M. Baba High-pressure freezing is a powerful tool for visualization of Schizosaccharomyces pombe cells: ultra-low temperature and low-voltage scanning electron microscopy and immunoelectron microscopy J. Electron Microsc. (Tokyo), April 1, 2006; 55(2): 75 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Q. Wu and T. D. Pollard Counting Cytokinesis Proteins Globally and Locally in Fission Yeast Science, October 14, 2005; 310(5746): 310 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Sirotkin, C. C. Beltzner, J.-B. Marchand, and T. D. Pollard Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast J. Cell Biol., August 15, 2005; 170(4): 637 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Rodal, L. Kozubowski, B. L. Goode, D. G. Drubin, and J. H. Hartwig Actin and Septin Ultrastructures at the Budding Yeast Cell Cortex Mol. Biol. Cell, January 1, 2005; 16(1): 372 - 384. [Abstract] [Full Text] [PDF]
|
|