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Journal of Electron Microscopy Advance Access originally published online on May 15, 2006
Journal of Electron Microscopy 2006 55(2):97-105; doi:10.1093/jmicro/dfl004
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Ultrastructural transformation of gastric parietal cells reverting from the active to the resting state of acid secretion revealed in isolated rat gastric mucosa model processed by high-pressure freezing

Akira Sawaguchi, Fumiyo Aoyama, Mitsuru Ohashi, Soyuki Ide and Tatsuo Suganuma*

Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki Miyazaki 889-1692, Japan

*To whom correspondence should be addressed. E-mail: suganumat{at}med.miyazaki-u.ac.jp

To elucidate a functional transformation of gastric parietal cells, we have newly developed an isolated rat gastric mucosa model whose parietal cells exhibited a reverting process from the active to the resting state of acid secretion. Briefly, the parietal cells were treated with cimetidine following prior stimulation of acid secretion in the model, and cryofixed by plunge freezing for light microscopy or high-pressure freezing for electron microscopy. As a result, immunohistochemistry of H+/K+-ATPase demonstrated a progressive translocation of H+/K+-ATPase from the apical to the cytoplasmic region. The ultrastructure of parietal cells at 5 min in the reverting phase was quite similar to that of maximally stimulated one. However, the apical microvilli of intracellular canaliculi (IC) changed bulbous by degrees, resulted in complete occlusion of IC at 60 min in the reverting phase. The apical membranes were subsequently internalized into the cytoplasm forming unique penta-laminar membranes. Interestingly, at 90 min in the reverting phase, the penta-laminar membranes formed a number of multilamellar autophagosomes that were intensely labeled for H+/K+-ATPase. Then, the parietal cells exhibited well-developed Golgi apparatus and lysosomal compartments involving the multilamellar membranes at 105 min, and mostly reverted to their resting conformation at 120 min in the reverting phase. Corresponding to the ultrastructural changes of microvilli, the immunohistochemistry of ezrin showed a dissociation of ezrin from the apical region at 30 min in the reverting phase. The present findings provide new insights into the functional transformation in gastric parietal cells reverting to their resting conformation.

Keywords     isolated gastric mucosa, parietal cell, transformation, reverting process, H+/K+-ATPase, high-pressure freezing

Received     25 January 2006, accepted 6 March 2006


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