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Journal of Electron Microscopy 2007 56(3):77-81; doi:10.1093/jmicro/dfm009
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Three-Dimensional Architecture of Virus-Packed Tubule

Sumie Katayama1, Taiyun Wei2, Toshihiro Omura2, Junichi Takagi1 and Kenji Iwasaki1,3,*

1 Institute for Protein Research, Laboratory of Protein Synthesis and Expression, Osaka University, 3-2 Yamada, Suita, Osaka 565-0871, Japan
2 Laboratory of Virology, National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan
3 Core Research for Evolution Science and Technology (CREST), Japan Science and Technology Agency, Japan

* To whom correspondence should be addressed. E-mail: ikenji{at}protein.osaka-u.ac.jp

When rice dwarf virus (RDV), a member of the Reoviridae family, infects leafhopper cells, formation of protruding tubules composed of nonstructural viral protein Pns10 can be observed. We examined the three-dimensional (3D) structure of these tubules containing RDV particles using electron tomography. The thin section of RDV-infected leafhopper vector cells in monolayers was subjected to double-tilt tomography. The tomographic 3D map provides a more reliable estimation of the real dimensions of the structure compared with the 2D image of the thin section. Docking of particle models made from atomic coordinates of RDV into the tomogram revealed that the inner diameter of the tubule was close to the outer diameter of the RDV particle. Fourier-transform of the reconstituted tubule image from the purified Pns10 protein in vitro revealed a helical structure of the tubule.

Keywords     tomography, virus, RDV

Received     31 January 2007, accepted 19 March 2007


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