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Journal of Electron Microscopy Advance Access published online on June 14, 2006
Journal of Electron Microscopy, doi:10.1093/jmicro/dfl015
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received December 23, 2005
Accepted May 8, 2006

Full-length paper: Biological

The visualization of hepatic vasculature by X-ray micro-computed tomography

Shalini Ananda 1 {dagger}, Valerie Marsden 1 {dagger}, Katrien Vekemans 2, Emine Korkmaz 1, Naomi Tsafnat 1, Lilian Soon 1, Allan Jones 1, and Filip Braet 1 *

1 Australian Key Centre for Microscopy and Microanalysis (AKCMM), Electron Microscope Unit, The University of Sydney, NSW 2006, Australia
2 Abdominal Transplant Surgery Department, Centre of Experimental Surgery and Anaesthesia, Catholic University of Leuven, Leuven 3000, Belgium

* To whom correspondence should be addressed.
Filip Braet, E-mail: filip.braet{at}emu.usyd.edu.au


  Abstract

In this study, X-ray micro-computed tomography (CT) was used to reconstruct the fine structure macro- and microvasculature in three dimensions in contrast-enhanced rat liver samples. The subsequent application in the experimental CC531s colorectal cancer model was concurrent with results obtained from confocal microscopy in earlier studies. The en bloc stains osmium tetroxide in combination with uranyl acetate provided an excellent contrasting result for hepatic tissue after a trial of several contrasting agents. X-ray micro-CT allowed us to image the large blood vessels together with the branching sinusoids of hepatic tissue in three dimensions. Furthermore, interruption of the microvasculature was noted when rats were injected with CC531s colorectal cancer cells indicating the presence of hepatic metastases.

Keywords: capillaries; colon cancer; CC531s; en bloc staining; liver; 3D-reconstruction; sinusoids.
{dagger}The first two authors as well as the last two authors contributed equally to this work.
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