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Journal of Electron Microscopy Advance Access published online on August 22, 2008
Journal of Electron Microscopy, doi:10.1093/jmicro/dfn015
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Comment on ‘Electron holography on dynamic motion of secondary electrons around sciatic nerve tissues’

Marco Beleggia1,* and Giulio Pozzi2

1 Institute for Materials Research, University of Leeds, Leeds LS2 9JT, UK
2 Dipartimento di Fisica, Università di Bologna, V. le B. Pichat 6/2, 40127 Bologna, Italy

* To whom correspondence should be addressed. E-mail: m.beleggia{at}leeds.ac.uk.

We present an alternative interpretation of the holographic phase dislocation loops revealed by Shindo et al. [J. Electron Microsc. 56(1): 1–5 (2007)] around a charged sample. Our interpretation does not involve the motion of secondary electrons around a charged object. It relates, instead, to fluctuating charges on the sample and to the resulting Moiré-type patterns in the hologram.

Keywords     electron holography, electrostatic potential, electron-optical phase shift, double-exposure holography, charging effects, charge fluctuations

Received     28 July 2008, accepted 11 August 2008


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